Scientific Papers
Julia Prigann, Rubens Tavora, Robert L Furler O’Brien, Ursula Schulze-Gahmen, Daniela Boehm, Nadia R Roan, Douglas F Nixon, Lishomwa C Ndhlovu, Susana Valente, Melanie Ott. Silencing the transcriptionally active HIV reservoir to improve treatment outcomes. Nature Microbiology. 2024 Sep 17. doi: 10.1038/s41564-024-01816-5. Online ahead of print.
Luisa P Mori, Michael J Corley, Andrew T McAuley, Alina Pang, Thomas Venables, Lishomwa C Ndhlovu, Matthew E Pipkin, Susana T Valente. Transcriptional and methylation outcomes of didehydro-cortistatin A use in HIV-1–infected CD4+ T cells. Life Sci Alliance. 2024 Oct; 7(10): e202402653. Published online 2024 Aug 1. doi: 10.26508/lsa.202402653
Wang H, Yuan Z, Pavel MA, Jablonski SM, Jablonski J, Hobson R, Valente S, Reddy CB, Hansen SB. The role of high cholesterol in SARS-CoV-2 infectivity. J Biol Chem. 2023 Jun;299(6):104763. doi: 10.1016/j.jbc.2023.104763. Epub 2023 Apr 28. PubMed PMID: 37119851; PubMed Central PMCID: PMC10140059.
Gibaut QMR, Mori LP, Valente ST. HIV-1 transcriptional modulation: novel host factors and prospective therapeutic strategies. Curr Opin HIV AIDS. 2023 Sep 1;18(5):264-272. doi: 10.1097/COH.0000000000000808. Epub 2023 Jul 17. Review. PubMed PMID: 37535041; PubMed Central PMCID: PMC10403276.
Lyons DE, Kumar P, Roan NR, Defechereux PA, Feschotte C, Lange UC, Murthy N, Sameshima P, Verdin E, Ake JA, Parsons MS, Nath A, Gianella S, Smith DM, Kallas EG, Villa TJ, Strange R, Mwesigwa B, Furler O’Brien RL, Nixon DF, Ndhlovu LC, Valente ST, Ott M. HIV-1 Remission: Accelerating the Path to Permanent HIV-1 Silencing. Viruses. 2023 Oct 28;15(11). doi: 10.3390/v15112171. PubMed PMID: 38005849; PubMed Central PMCID: PMC10674359.
Mallorson R, Miyagi E, Kao S, Sukegawa S, Saito H, Fabryova H, Morellatto Ruggieri L, Mediouni S, Valente ST, Strebel K. Transcriptional regulation of the HIV-1 inhibitory factor human mannose receptor 1 by the myeloid-specific transcription factor PU.1. J Virol. 2024 Jan 23;98(1):e0170223. doi: 10.1128/jvi.01702-23. Epub 2023 Dec 11. PubMed PMID: 38078733; PubMed Central PMCID: PMC10804955.
Ling L, Leda AR, Begum N, Spagnuolo R, Wahl A, Garcia J, Valente ST. Loss of In Vivo Replication Fitness of HIV-1 Variants Resistant to the Tat Inhibitor, dCA. Viruses. 2023 April; 15(4). doi: 10.3390/v15040950.
Li C, Mori LP, Lyu S, Bronson R, Getzler AJ, Pipkin ME, Valente ST. The chaperone protein p32 stabilizes HIV-1 Tat and strengthens the p-TEFb/RNAPII/TAR complex promoting HIV transcription elongation. Proc Natl Acad Sci U S A. 2023 Jan 3;120(1):e2217476120. doi: 10.1073/pnas.2217476120. Epub 2022 Dec 30. PubMed PMID: 36584296; PubMed Central PMCID: PMC9910500.
Vartak R, Jablonski J, Deore B, Mediouni S, Sanhueza CA, Valente ST, Patel K. Bictegravir nanomicelles and anionic pullulan loaded vaginal film: Dual mechanistic pre-exposure prophylaxis (PrEP) for HIV. Int J Biol Macromol. 2022 Nov 30;221:416-425. doi: 10.1016/j.ijbiomac.2022.08.211. Epub 2022 Sep 6. PubMed PMID: 36075305.
Yuan Z, Pavel MA, Wang H, Kwachukwu JC, Mediouni S, Jablonski JA, Nettles KW, Reddy CB, Valente ST, Hansen SB. Hydroxychloroquine blocks SARS-CoV-2 entry into the endocytic pathway in mammalian cell culture. Commun Biol. 2022 Sep 14;5(1):958. doi: 10.1038/s42003-022-03841-8. PubMed PMID: 36104427; PubMed Central PMCID: PMC9472185.
Mediouni S, Lyu S, Schader SM, Valente ST. Forging a Functional Cure for HIV: Transcription Regulators and Inhibitors. Viruses. 2022 Sep 7;14(9). doi: 10.3390/v14091980. Review. PubMed PMID: 36146786; PubMed Central PMCID: PMC9502519.
Mediouni S, Mou H, Otsuka Y, Jablonski JA, Adcock RS, Batra L, Chung D, Rood C, S. de Vera IA, Rahaim R, Ullah S, Yu X, Nguyen T, Hull M, Chen E, Bannister TD, Scampavia L, Farzan M*, Valente ST*, Spicer TP*. Identification of Potent Small Molecules Inhibitors of SARS-CoV-2 Entry. *Equal contribution. 2022. SLAS Discovery. 2022 Jan;27(1):8-19. doi: 10.1016/j.slasd.2021.10.012. Epub 2021 Oct 23 Journal editors top 10 paper for 2022
Deeks SG, Lewin SR, Valente ST, et al. International AIDS Society global scientific strategy: towards an HIV cure 2021. Nat Med. 2021 Dec;27(12):2085-2098. doi: 10.1038/s41591-021-01590-5. Epub 2021 Dec 1.
Premeaux TA, Mediouni S, Leda A, Furler RL, Valente ST, Fine HA, Nixon DF, and Ndhlovu LC. Next Generation Human Cerebral Organoids as Powerful tools to Advance NeuroHIV Research. 2021. mBio. PubMed PMID: 34253056. DOI:10.1128/mBio.00680-21.
Swarnkar S, Avchalumov Y, Espadas I, Grinman E, Liu X, Raveendra BL, Zuca A, Mediouni S, Valente ST, Page D, Miller K, Puthanveettil SV. The Molecular Motor Protein KIF5C Mediates Structural Plasticity and Long-Term Memory by Constraining Local Translation. 2021. Cell Reports. 2021 July 13, vol36, Issue 2. PedMed PMID: 34260917. PubMed Central PMCID: PMC8319835. doi: 10.1016/j.celrep.2021.109369.
Li C, Mori L and Valente ST. The block-and-lock strategy for Human Immunodeficiency Virus Cure: Lessons Learned from didehydro-Cortistatin A. Journal of Infectious Diseases. 2021 Feb15; 223:46-53. PubMed PMID: 33586776. PubMed Central PMCID: PMC7883021. DOI:10.1093/infdis/jiaa681.
Moranguinho I and Valente ST. Block-and-Lock: new horizons for a cure for HIV. Viruses. 2020 Dec15.12(2):1443. PubmEd PMID: 33334019. PubMed Central PMCID: PMC7765451.
Mori L, Jenike K, Yeh YJ, Lacombe B, Li C, Getzler A, Mediouni S, Cameron M, Pipkin M, Ho YC, Ramirez BC, Valente S. The XPB Subunit of the TFIIH Complex Plays a Critical Role in HIV-1 Transcription and XPB Inhibition by Spironolactone Prevents HIV-1 Reactivation from Latency. Journal of Virology. 2020 Nov 25;. doi: 10.1128/JVI.01247-20. PubMed PMID: 33239456; PubMed Central PMCID: PMC7851559.
Vartak R, Patki M, Menon S, Jablonski J, Mediouni S, Fu Y, Valente ST, Billack B, Patel K. β-cyclodextrin polymer/Soluplus® encapsulated Ebselen ternary complex (EβpolySol) as a potential therapy for vaginal candidiasis and pre-exposure prophylactic for HIV. Int J Pharm. 2020 Nov 15;589:119863. doi: 10.1016/j.ijpharm.2020.119863. Epub 2020 Sep 8. PubMed PMID: 32911046.
Patki M, Vartak R, Jablonski J, Mediouni S, Gandhi T, Fu Y, Cetindag E, Dave R, Valente ST, Patel K. Efavirenz Nanomicelles Loaded Vaginal Film (EZ film) for Preexposure Prophylaxis (PrEP) of HIV. 2020. Colloids and Surfaces B: Biointerfaces. 2020 Oct; 194:111174. DOI:10.1016/j.colsurfb.2020.111174. PubMed: PMID: 32540766.
Mediouni S, Jablonski JA, Tsuda S, Barsamian A, Kessing C, Richard A, Biswas A, Toledo F, Andrade VM, Even Y, Stevenson M, Tellinghuisen T, Choe H, Cameron M, Bannister TD, Valente ST. Oregano Oil and Its Principal Component, Carvacrol, Inhibit HIV-1 Fusion into Target Cells. Journal of Virology. 2020 Jul 16;94(15). doi: 10.1128/JVI.00147-20. Print 2020 Jul 16. PubMed PMID: 32461309; PubMed Central PMCID: PMC7375364.
Mori L and Valente S. Key Players in HIV-1 Transcriptional Regulation: Targets for a Functional Cure. Viruses. 2020, 12(5), 529; doi:10.3390/v12050529. PubMed PMID: 32403278. PubMed Central: PMC7291152.
Mousseau G, Aneja R, Clementz MA, Kessing CF, Jablonski J, Thenin-Houssier S, Mediouni S, Nagarsheth N, Valente ST. Resistance to the Tat inhibitor didehydro-Cortistatin A is mediated by heightened basal HIV-1 transcription. 2019. mBio. 2019 Jul 2;10(4):e01750-18. DOI: 10.1128/mBio.01750-18. PubMed PMID: 31266880. PubMed Central PMCID: PMC6606815.
Mediouni S, Kessing C, Thenin-Houssier S, Clementz M, Mousseau G, de Vera IM, Kovak MD, Jablonski J, Kojetin DJ, Evans D & Valente ST. The Tat inhibitor didehydro-Cortistatin A suppresses SIV replication and reactivation. FASEB Journal. 2019 Jul;33(7):8280-8293. PubMed PMID: 31021670. PubMed Central PMCID: PMC6593873.
Takata H, Kessing C, Sy A, Sciumbata J, Lima N, Mori L, Jones BR, Chomont N, Miachael NL, Valente ST and Trautmann L. Modeling HIV-1 latency using primary CD4+T cells from Virally Suppressed HIV-1 infected Individuals on Antiretroviral Therapy. Journal of Virology. 2019 March 15:93(11):e02248-18. PubMed PMID: 30918073. PubMed Central PMCID: PMC6532074. DOI: 10.1128/JVI.02248-18.
Li C, Mousseau G, Valente ST. Tat inhibition by didehydro-Cortistatin A promotes heterochromatin formation at the HIV-1 Long Terminal repeat. Epigenetics & Chromatin. 2019 April 16;12(1):23. doi: 10.1186/s13072-019-0267-8.PubMed PMID: 30992052. PubMed Central PMCID: PMC6466689.
Mediouni S, Chinthalapudi K, Ekka M, Usui I, Jablonski J, Clementz M, Mousseau G, Nowak J, Macherla VR, Beverage JN, Esquenazi E, Baran P, Loret EP, Nettles K, Maiti S, Izard T, Valente ST. Didehydro-Cortistatin A inhibits the activity of HIV-1 by specifically binding to the unstructured basic region of Tat. mBio. 2019 Feb 5;10(1). pii: e02662-18. doi: 10.1128/mBio.02662-18. PubMed PMDI: 30723126. PubMed Central PMCID: PMC6368365.
Mediouni S, Jablonski JA, Tsuda S, Richard A, Kessing CF, Andrade MV, Biswas A, Even Y, Tellinghuisen T, Choe H, Cameron M, Stevenson M, Valente ST. Potent suppression of HIV-1 cell attachment by Kudzu root extract. Retrovirology. 2018; Sep 20:15(1):64.doi: 10.1186/s12977-018-0446-x. PubMed PMID: 30236131. PubMed Central PMCID: PMC6149077.
Kessing CF, Nixon CC, Li C, Tsai PM, Takata H, Mousseau G, Ho PT, Honeycutt JB, Fallahi M, Trautmann L, Garcia JV, Valente ST. In vivo suppression of HIV rebound by didehydro-Cortistatin A, a novel “block-and-lock” strategy for HIV-1 Treatment. Cell Reports. 2017; 21(3):600-611. PubMed PMID: 29045830; NIHMSID: NIHMS910101; PubMed Central PMCID: PMC5653276.
Mousseau G. & Valente ST. Role of Host Factors on the Regulation of Tat-Mediated HIV-1 Transcription. Current Pharmaceutical Design. 2017; 23(28):4079-4090. PubMed PMID: 28641539; NIHMSID: NIHMS924849; PubMed Central PMCID: PMC5731639. doi : 10.2174/1381612823666170622104.
Wu H, Mousseau G, Mediouni S, Valente ST, Kodadek T. Cell-permeable peptides containing cycloalanine Residues. Angewandte Chem In Ed Engl. 2016;55(41):12637-42. PubMed PMID: 27529332; NIHMSID: NIHMS833797; PubMed Central PMCID: PMC5220248.
Deeks SG, Lewin SR, Ross AL, Ananworanich J, Benkirane M, Cannon P, Chomont N, Douek D, Lifson JD, Lo YR, Kuritzkes D, Margolis D, Mellors J, Persaud D, Tucker JD, Barre-Sinoussi F; International AIDS Society Towards a Cure Working Group, Alter G, Auerbach J, Autran B, Barouch DH, Behrens G, Cavazzana M, Chen Z, Cohen ÉA, Corbelli GM, Eholié S, Eyal N, Fidler S, Garcia L, Grossman C, Henderson G, Henrich TJ, Jefferys R, Kiem HP, McCune J, Moodley K, Newman PA, Nijhuis M, Nsubuga MS, Ott M, Palmer S, Richman D, Saez-Cirion A, Sharp M, Siliciano J, Silvestri G, Singh J, Spire B, Taylor J, Tolstrup M, Valente S, van Lunzen J, Walensky R, Wilson I, Zack J. International AIDS Society global scientific strategy: towards an HIV cure 2016. Nat Med. 2016: 22(8):839-50. PubMed PMID: 27400264; NIHMSID: NIHMS848236; PubMed Central PMCID: PMC5322797.
Thenin-Houssier S, de Vera IM, Pedro-Rosa L, Brady A, Richard A, Konnick B, Opp S, Buffone C, Fuhrmann J, Kota S, Billack B, Pietka-Ottlik M, Tellinghuisen T, Choe H, Spicer T, Scampavia L, Diaz-Griffero F, Kojetin DJ & Valente ST. Ebselen, a small molecule capsid-inhibitor of HIV-1 replication. Antimicrob Agents Chemother. 2016; 60(4):2195-208. PubMed PMID: 26810656; PubMed Central PMCID: PMC4808204.
Mousseau G & Valente ST. Didehydro-Cortistatin A: A new player in HIV-therapy? Expert Rev Anti Infect Ther. 2016;14(2):145-8. PubMed PMID: 26581953; NIHMSID: NIHMS764000; PubMed Central PMCID: PMC4793404. doi: 10.1586/14787210.2016.1122525.
Thenin-Houssier S & Valente ST. HIV-1 capsid inhibitors as antiretroviral agents. Current HIV Research. 2016; 14(3):270-82. PubMed PMID: 26957201; NIHMSID: NIHMS740513; PubMed Central PMCID: PMC4785820. doi: 10.2174/1570162×14999160224103555.
Mediouni S, Marcondes MCG, Miller C, McLaughlin JP, Valente ST. The cross-Talk of HIV-1 Tat and Methamphetamine in HIV associated neurocognitive disorders. Front. Microbiol. 2015; 6:1164. PubMed PMID: 26557111; PubMed Central PMCID: PMC4615951.
Mousseau G., Kessing, C., Fromentin R., Trautmann, L., Chomont N. and Valente ST. The Tat inhibitor didehydro-Cortistatin A prevents HIV-1 reactivation from latency. mBio. 2015; 6 (4):e00465. PubMed PMID: 26152583; PubMed Central PMCID: PMC4495168. Editors’ Pick.
Mediouni S, Paris JJ, Jablonski J, Clementz M, Thenin-Houssier S, McLaughlin JP, Valente ST. Didehydro-Cortistatin A inhibits HIV-1 Tat mediated Neuroinflammation and Prevents Potentiation of Cocaine Reward in Tat Transgenic Mice. Current HIV Research. 2015; 13(1):64-79. PubMed PMID: 25613133; NIHMSID: NIHMS679919; PubMed Central PMCID: PMC4416414. doi:10.2174/1570162X13666150121111548.
Jablonski J, Clementz M, Ryan K, Valente ST. Analysis of RNA processing reactions using cell free systems: 3′ end cleavage of pre-mRNA substrates in vitro. JoVE. 2014; 3(87):51309. PubMed PMID: 24835792; NIHMSID: NIHMS533499; PubMed Central PMCID: PMC4030693. JoVE Video link
Mousseau G & Valente ST. Strategies to Block HIV Transcription: Focus on Small Molecule Tat Inhibitors. Biology. 2012; 1(3), 668-697. PubMed PMID: 24832514; PubMed Central PMCID: PMC4009808.
Mousseau G, Clementz M, Bakeman W, Nagarsheth N, Cameron M, Shi J, Baran P, Fromentin R, Chomont N & Valente ST. An analog of the Natural Steroidal Alkaloid Cortistatin A potently suppresses Tat-dependent HIV transcription. Cell Host & Microbe. 2012; 12(1):97-108. PubMed PMID: 22817991; NIHMSID: NIHMS390360; PubMed Central PMCID: PMC3403716.
Valente ST*, Gilmartin GM, Venkataraman K, Arriagada G & Goff SP. HIV-1 mRNA 3’ end processing is distinctively regulated by eIF3f, CDK11, and splice factor 9G8. Molecular Cell. 2009; 23;36(2):279-89 PubMed PMID: 19854136; NIHMSID: NIHMS156755; PubMed Central PMCID: PMC3068534. *corresponding author.
Valente ST, Gilmartin GM, Mott C, Falkard B & Goff SP. Inhibition of HIV-1 replication by eIF3f. Proc Natl Acad Sci USA. 2009; 106(11):4071-4078. PubMed PMID: 19237569; PubMed Central PMCID: PMC2646627.
Naghavi MH, Valente S, Hatziioannou T, de Los Santos K, Wen Y, Mott C, Gundersen GG & Goff SP. Moesin regulates stable microtubule formation and limits retroviral infection in cultured cells. EMBO Journal. 2007; 26:41-52. PubMed PMID: 17170707; PubMed Central PMCID: PMC1782362.
Valente S & Goff SP. Inhibition of HIV-1 Gene Expression by a Fragment of hnRNP U. Molecular Cell. 2006; 23(4):597-605. PubMed PMID: 16916646.
Hazan U, Romero IA, Cancello R, Valente S, Perrin V, Mariot V, Dumonceaux J, Gerhardt CC, Strosberg AD, Couraud PO & Pietri-Rouxel F. Human adipose cells express CD4, CXCR4, and CCR5 [corrected] receptors: a new target cell type for the immunodeficiency virus-1? FASEB Journal. 2002; 16(10):1254-1256. PubMed PMID: 12153994.
Valente ST, Chanel C, Dumonceaux J, Olivier R, Marullo S, Briand P & Hazan U. CXCR4 is down-regulated in cells infected with the CD4-independent X4 human immunodeficiency virus type 1 isolate m7NDK. Journal of Virology. 2001; 75:439-447. PubMed PMID: 11119612; PubMed Central PMCID: PMC113936.
Dumonceaux J, Chanel C, Valente S, Quivet L, Briand P & Hazan U. Mutations in the env gene of human immunodeficiency virus type 1 NDK isolates and the use of African green monkey CXCR4 as a co-receptor in COS-7 cells. Journal of General Virology. 1999; 80 (8):1975-1982. PubMed PMID: 10466793.
Book Chapters
Mori L and Valente ST. Chapter 6. Cure and long-term remission strategies. Methods in Molecular Virology, HIV reservoirs: modeling, quantification, and approached to a cure. 2022. HIV Reservoirs. 2022;2407:391-428. doi: 10.1007/978-1-0716-1871-4_26.
Na M, Valente ST & Ryan K. Optimizing In Vitro Pre-mRNA 3′ Cleavage Efficiency: Reconstitution from Anion-Exchange Separated HeLa Cleavage Factors and from Adherent HeLa Cell Nuclear Extract. Methods Mol Biol. 2017; 1507:179-198. PubMed PMID: 27832541. doi: https://doi.org/10.1007/978-1-4939-6518-2_14
Mousseau G, Mediouni S & Valente ST. Targeting HIV transcription: The Quest for a Functional Cure. Current Topics in Microbiology and Immunology. Series on novel and future small molecule treatments for HIV; 2015; 389:121-45. PubMed PMID: 25731772; NIHMSID: NIHMS680213; PubMed Central PMCID: PMC4409574.doi:10.1007/82_2015_435. https://pubmed.ncbi.nlm.nih.gov/25731772/
Valente ST & Goff SP. Somatic Cell Genetic Analyses to Identify HIV-1 Host Restriction Factors.Methods Molecular Biology. 2009; 485:235-255. PubMed PMID: 19020830; NIHMSID: NIHMS518013; PubMed Central PMCID: PMC3804074. doi: https://doi.org/10.1007/978-1-59745-170-3_17
